CHROMATOGRAPHY, Vol. 34 (2013), No. 1, pp. -41

Superficially porous particle (core shell particle) has been available as an alternative to using sub-2um particle for HPLC or UHPLC. Core shell particles are composed of a 1.2 to 1.9 μm solid core encircled a 0.25 μm to 0.5 μm porous layer. Especially a 2.6 μm or 2.7 μm core shell particle shows a half back pressure and almost the same efficiency to compare with sub-2 μm particle because of a large particle and reducing mass transfer due to a thin porous silica layer. In this study, separation characteristics of a core shell particle C18 column was compared with 2 μm and sub 2 μm particle C18 columns and the availability of a core shell particle C18 column for a routine analysis instead of a 5 μm particle column was evaluated.