Original

Influences of Analyte Injection Volumes and Concentrations on Capillary Chromatography Based on Tube Radial Distribution of Carrier Solvents under Laminar Flow Conditions

Yusuke Tanigawa, Naoya Jinno, Masahiko Hashimoto and Kazuhiko Tsukagoshi

Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan

Abstract:

A capillary chromatography called the tube radial distribution chromatography (TRDC) system has been developed using an open capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture carrier solution. In this study, we examined the effects of the analyte injection volume (injection time) and concentration on the chromatograms in the TRDC system using a fused-silica capillary tube (50 μm i.d. and 100 cm effective length) and a water-acetonitrile-ethyl acetate mixture carrier solution (volume ratio, 3: 8: 4). Analyte solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid (1 mM each) were injected into the system with various injection times of 10-1500 s from a height of 20 cm by gravity. They were separated and detected in this order with good reproducibility up to an injection time of 150 s. The analyte solutions (0.075-3.0 mM) were analyzed with the definite injection time of 30 s from a height of 20 cm by gravity. They were separated and detected with a baseline separation and their calibration curves showed linearity up to 1.5 mM. It was confirmed that the TRDC system worked well as a quantitative analysis.

Keywords: chromatography, tube radial distribution, laminar flow conditions, analyte injection volumes, analyte concentration

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