CHROMATOGRAPHY, Vol. 32 (2011), No. 2, pp. 73-79
Technical Review
逆相クロマトグラフィー用シリカ系カラム
Silica-Based Column for Reversed Phase Chromatography
須藤良久、坂牧寛
Yoshihisa Sudo, Hiroshi Sakamaki
Chemicals Evaluation and Research Institute, Japan
1600 Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama 345-0043 Japan
Abstract:
The column is the heart of the liquid chromatography system. The silica-based column for revered phase chromatography is used most
widely. We review this column concerning the characteristics of totally porous silica gel, the synthesis and the characteristics of octadecylsilylation
of silica gel and the methods and the importance of end-capping of residual silanol groups. In particular, we introduce the endcapping
using high-emperature silylation in detail.
Keywords:
silica-based column, reversed phase chromatography, silica gel, residual silanol group, end-capping, high-temperature silylation
