Technical Note

Simple Method for Determining Mitotane Concentration in Human Blood Plasma

Masako Nakazawa¹, Mari Egawa², Toshio Tanaka², and Ritsuo Aiyama¹

¹Yakult Honsha Co. Ltd., Medical Development Department, 1-12-5 Owada-machi, Hachioji, Tokyo 192-0045, Japan
²Shimadzu Techno-Research, Inc., 2-13, Nishinokyo-Sanjyobocho, Nakagyo-ku, Kyoto 604-8435, Japan

Abstract:

We developed a simple method for determining mitotane concentration in a patient’s plasma using a conventional HPLC instrument equipped with an ultraviolet-visible photometer and a stainless steel column packed with octadecylsilanized silica gel. The method was validated in terms of specificity, linearity, precision, accuracy, quantitation limit, and stability of test solutions in the concentration range of 0.2 to 40 μg/mL of mitotane in plasma samples.

Keywords: mitotane, Opeprim, plasma concentration, dichlorodiphenyldichloroethane, HPLC, validation.

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