CHROMATOGRAPHY, Vol. 31 (2010), No. 1, pp. -31

逆相分取HPLCを用いる効率的な精製方法
Effective preparative purification method for peptides and proteins using silica based reversed phase packing materials

表正克、山下智恵、森下潔、小路庸小、栗山尚浩
Masakatsu Omote, Chie Yamashita, Kiyoshi Morishita, Noriko Shoji, Naohiro Kuriyama

Reversed phase HPLC is an essential tool for analytical and preparative separation of peptides and proteins. Purification in preparative scale, it is important to choose optimum pore size and ligand. We discuss the difference between analytical and preparative HPLC, and show actual method developments effectively in preparative scale. We also show chemically durable packing material, named Exphere series which is suitable for peptides separation. The material is using complete sphere and mono dispersed silica which enables higher mechanical strength than conventional gel in repacking evaluation.