Focusing Review

Development of novel fluorescence derivatization systems enabling highly sensitive and selective detection of biological compounds

Kenichiro Todoroki

Faculty of Pharmaceutical Sciences, Fukuoka University
8-19-1 Nanakuma, Johnan-ku, Fukuoka 814-0180, Japan

Abstract:

A fluorescence derivatization-liquid chromatography (LC) method is a powerful tool for the analysis of bioactive compounds with high sensitivity and selectivity. In this paper, the author reviews the development of the following three types of novel fluorescence derivatization-LC analytical systems: (1) simultaneous LC analysis of melatonin and its related compounds through post-column electrochemical demethylation and fluorescence derivatization, (2) LC analysis of 5-hydroxyindoles based on fluorescence derivatization by online pre-column photocatalytic oxidation with benzylamine, and (3) reagent peak-free LC analysis for aliphatic amines and amino acids using F-trap pyrene as a fluorous tag-bound F-trap fluorescence derivatization reagent. The authors have also successfully applied these systems to biological and pharmaceutical analyses.

Keywords: Fluorescence derivatization, Pinpoint targeting reagent, Electrochemical oxidation, Photocatalytic oxidation, Fluorous separation, F-trap fluorescence derivatization

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