Focusing Review

HILIC separation of glycans and glycopeptides

Kisaburo Deguchi

Analytical Systems Design Department, Hitachi High-Technologies, Co. 882 Ichige, Hitachinaka, Ibaraki, 312-8504, Japan

Abstract:

Glycosylation is one of the most common post.translational modifications of proteins. It is related to numerous biological processes. N and O-glycans attached to proteins make up some of the most complex and challenging samples for chromatography because of the structural diversity of glycans (composition, sequence, anomeric character, linkage position, and branching pattern) and, as a result, the existence of many isomers. This review addresses the recent progress of the separation of glycans and glycopeptides, focusing on hydrophilic interaction chromatography (HILIC) and simply discusses the retention mechanism of neutral and sialylated glycans on a ZIC-HILIC column

Keywords: glycans, glycopeptides, HILIC separation, ZIC-HILIC column, mass spectrometry

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