Focusing Review

A Perspective of Hydrophilic Interaction Chromatography-Development and the Characteristics of the separation mode

Tohru Ikegami, Hirotaka Takubo, Nobuo Tanaka

Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585 Japan

Abstract:

Hydrophilic interaction chromatography (HILIC) has been focusing attentions of chromatographers in these years, because it is important and useful for the separation of highly polar analytes such as pharmaceutical drugs, neurotransmitters, nucleosides, nucleotides, amino acids, peptides, proteins, oligosaccharides, carbohydrates, and so on. Aqueous organic solvents are used as mobile phases on more polar stationary phases in the HILIC mode separation. A variety of stationary phases including bare silica, and silica phases modified with amino, amide, zwitterionic functional group, polyols including saccharides and other polar groups are used in the separation mode. This review discusses the variation and the historical view of the stationary phases, and column efficiency of HILIC stationary phases in relation to solute and stationary phase structures to provide a perspective of HILIC type stationary phases, and their separation efficiencies and retention tendencies.

Keywords:

[Full Text PDF]