Focusing Review

Development of Precise Chromatographic Separation, Preparation and Detection Tools for Biomedical Use

Kiyoshi Zaitsu

Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

Abstract:

This review describes the author’s various developed analytical tools (reagents and methods) for the precise chromatographic separation, preparation and determination of trace amounts of biologically important compounds in mammals. Especially, the development of tools combining highly sensitive fluorescence/chemiluminescence detection and highly selective HPLC are my central objective as in the following research studies: (1) fluorescent azole formation for the determination of aromatic aldehydes and catecholamines, (2) new hetero-bifunctional reagents for protein-protein conjugation, (3) fluorescent aminopyrazine reagent for the determination of saccharides (4) non-radioisotopic assay of insulin using insulin labeled at a selective position, (5) new concept of detachable amino-labeling reagents, which contain an acid anhydride moiety (6) highly sensitive HPLC for the determination of melatonin, and (7) highly sensitive HPLC of D-amino acids.

Keywords: HPLC, fluorescence, azole, hetero-bifunctional reagent, aminopyrazine, melatonin, D-amino acid

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