CHROMATOGRAPHY, Vol. 28 (2007), No. 3, pp. 131-135
Original
Development of a Solvent-Saving Direct.Pumping Recycle Chromatographic System and Its Application to the Separation of Deuterated Benzenes in Liquid Chromatography
Zarina Aspanut, Yuko Suzuki, Lee Wah Lim and Toyohide Takeuchi＊
Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
Abstract:
A closed.loop direct.pumping recycle chromatography (RC) system was examined for analytical scale to achieve high resolution in high-performance liquid chromatography (HPLC), where a 6-way switching valve was switched after the system had been stabilized, resulting in no more consumption of additional mobile phase. The extra-column band broadening effect was minimized by using an HPLC pump equipped with small-discharging.volume plungers, low-dead-volume devices and connecting tubes. The plate number achieved was linear to the number of cycle, and it increased by more than 10,000 per cycle using a 25-cm separation column packed with 5-μm reversed-phase materials. The mobile phase conditions were optimized in terms of analysis time, and the present RC system was applied to the separation of
benzene and deuterated benzene isomers.
Keywords:
HPLC, Recycle chromatography, Solvent-saving direct-pumping system, High resolution, Deuterated benzenes
