Technical Review

超高速液体クロマトグラフィーシステムX-LC^TM（Extreme Liquid Chromatography System）の特長とアミノ酸分析への応用
Feature of Extreme Liquid Chromatography System^TM and Application to Ultra High−Speed Separation of Amino Acids

坊之下雅夫，佐藤泰世，宮路敏彦，岩谷敬仁，山口高歩，東館栄，齋藤宗雄
Masao Bounoshita, Yasuyo Sato, Toshihiko Miyaji, Keijin Iwaya, Higashidate Sakae, Takayuki Yamaguchi and Muneo Saito

JASCO Corporation, 2967−5 Ishikawa−cho, Hachioji−shi, Tokyo, 192−8537 Japan

Abstract:

Ultra high−speed liquid chromatography has become increasingly popular in HPLC. In general, conventional HPLC separation has been performed on columns packed with 5 μm particles. However, conventional HPLC requires a long analysis time and a large amounts of solvents. In case of ultra high−speed liquid chromatography, shorter columns typically 50−100 mm in length and 2 mm I.D. packed with ultra microparticles, of which particle size is less than 2 μm, are used. X−LC (extreme liquid chromatograph) offers higher efficiency and sensitivity, and reduction of organic solvents without sacrificing separation efficiency. We outline the new generation HPLC equipment X−LC that can be used for ultra high−speed liquid chromatographic separation. We also discuss the analysis of amino acids using the X−LC system, utilizing a combination of precolumn derivatization with OPA (orthophthalic aldehyde) and separation on a 2−μm dia C18 column with fluorescence detection.

Keywords: Ultra high−speed liquid chromatography, X−LC, Extreme liquid chromatography, Amino acids, precolumn derivatization, OPA, preheat−coil, High−Throughput

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