CHROMATOGRAPHY
CHROMATOGRAPHY, Vol. 28 (2007), No. 2, pp. 75-79
Technical Review
液体クロマトグラフィーにおける超高速・超高分離技術の確立
New Foundation of Ultra−High−Speed and Separation Technologies on Liquid Chromatography
江崎達哉,田中直美
Tatsuya Ezaki, Naomi Tanaka
日本ウォーターズ株式会社東京都品川区北品川1-3-12
Abstract:
The resolution needs to be compromised if the run time needs to be shorted in the traditional HPLC analysis as fast LC. To shorten the run time, the column length should be shorter and the mobile phase flow rate should be faster. But the resolution can be lower at the same time. "Van Deemter's law" explains the resolution can be maximized using theoretically suitable mobile phase flow rate. Also it shows the smaller column particle brings higher resolution. But it was so difficult to develop the LC columns and the hardware, which had higher resistance to pressure. Waters BEH technology and UPLC system technology made such ideal separation technology possible.
Keywords: Ultra Performance LC, UPLC, HPLC, Fast LC, UV, Van Deemter's law