Technical Review

Effect of Impurities in Ultra−pure Water on Trace Organism Analysis by HPLC

Yoshifumi Kuroki

ORGANO CORPORATION, 2−8, Shin−suna, 1−chome, Koto−ku, Tokyo, 136−8631

Abstract:

The quality of water has a significant influence on instrumental analyses.
In the microanalysis of trace organisms by HPLC or LC/MS, remaining organisms in the blank water should be decreased to less than 5 μg C/ L(TOC). Today we can easily obtain the ultra−pure water of such a high quality for LC/MS using a water purifier with high performance.
However, we often make mistakes in the usage of ultra−pure water and also in the operation of a water purifier.
The manner to use ultra−pure water is quite important as well as the performance of the water purifier. It is necessary to make a standard method to use ultra−pure water for trace organisms analysis.

Keywords: HPLC, LC/MS, Ultra−pure water, TOC, Impurity, Contamination, Water quality, trace organisms.

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