CHROMATOGRAPHY, Vol. 27 (2006), No. 2, pp. 69-72
Technical Review
LC / Linear Ion Trap−Time of Flight Mass Spectrometer for Protein Analysis Using Nano LC With Dual Exchange Gradient System
Izumi Ogata*, Kimihiko Ishii, Eri Yamashita, Shinji Yoshioka and Yasushi Terui
Hitachi High−Technologies Corporation, 882, Ichige, Hitachinaka, Ibaraki, 312−8504, Japan
Abstract:
“NanoFrontier LD”, a new LC/Linear Ion Trap (LIT)− Time of Flight (TOF) MS for protein analysis has developed by Hitachi High−Technologies
Corp. One of its remarkable features is NanoLC with Dual Exchange Gradient System (DEGS). DEGS offers great reproducibility of
nano−flow gradient elution. Connecting NanoLC and LIT−TOF MS which MSn analysis with high mass accuracy is available, Information
Based Acquisition (IBA) is also available. With IBA, the efficiency of protein identification by MS/MS improves.
Keywords:
LC/MS ; nanoLC ; proteomics
