CHROMATOGRAPHY, Vol. 27 (2006), No. 1, pp. 19-26

グアニジン変性したタンパク質のハイドロキシアパタイトクロマトグラフィー ——１．グアニジン含有リン酸緩衝液溶離系——
Hydroxyapatite chromatography of guanidine denatured proteins ——1. Guanidine containing phosphate buffer system——

吉武朋彦^1*、小林伸太郎¹、小川哲朗¹、奥山典生²
Tomohiko Yoshitake^1* , Shintaro Kobayashi¹ , Tetsuro Ogawa¹ and Tsuneo Okuyama²

¹New Ceramics Division, PENTAX Corporation 2-36-9, Maeno-cho, Itabashi-ku, Tokyo, 174-8639, Japan
²Protein Technos institute 99-7 Shimokawa-iri, Atugi-shi, Kanagawa-ken, 243-0206, Japan

Elution behaviors of guanidine denatured proteins on ceramic hydroxyapatite column with 2 M guanidine containing phosphate buffer system were investigated. In phosphate buffer system, the retention time of proteins was increased with the positive charge of the proteins. On the other hand, in guanidine containing phosphate buffer system, the retention time of the proteins decreased with the positive charge of the proteins. Guanidine denatured acidic and neutral proteins were retained on the column, however the most of the basic proteins were not retained on the column, except cytochrome-c. In sulfonate type cation.exchange chromatography as comparison, showed no retention of cytochrome-c with 2 M guanidine buffer system.