CHROMATOGRAPHY, Vol. 26 (2005), No. 3, pp. 101-104
Technical Note
Application of Monolithic Silica Column for HPLC Separation of Estrogens
Tatsuhito Mizuguchi, Chie Ogasawara and Kazutake Shimada*
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology,
Kanazawa University, Kakuma-machi, Kanazawa 920-1192 Japan
Abstract:
A monolithic silica column was used for the HPLC separation of estrogens, and its chromatographic behavior was compared to that of three
particle-packed columns (conventional columns). Generally speaking, the monolithic silica column provided a satisfactory separation with a
smaller retention factor than those obtained from the conventional columns. The application of the monolithic silica column for separation of
the formed products during the in vitro and photocatalytic degradation experiments using estrogen as a substrate was described.
Keywords:
Monolithic Silica Column/ HPLC/ Fast Separation/ Estrogen/ In Vitro Experiment/ Photocatalytic Degradation Experiment
