CHROMATOGRAPHY, Vol. 26 (2005), No. 3, pp. 95-99
Focusing Review
Development of an Analytical Method for Catecholamines with
HPLC-Chemiluminescence Detection and its Application
Kimiko Yoshida and Hitoshi Uemori
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Abstract:
Catecholamines play important roles as neurotransmitters and hormones, and are metabolized by catechol-O-methyltransferase (COMT) and
inactivated. We have developed the highly selective and sensitive determination methods for catecholamines (and their 3-O-methyl metabolites)
with high-performance liquid chromatography-peroxyoxalate chemiluminescence reaction detection. The developed methods were applicable
to the clarification of the roles of catecholamines and their 3-O-methyl metabolites in blood pressure regulation.
Keywords:
peroxyoxalate chemiluminescence; high-performance liquid chromatography (HPLC); catecholamines; catechol-O-methyltransferase
(COMT); blood pressure
