Technical Review

HPLC analysis of Tetracyclines in Shrimp

Kimiko Yoshida and Hitoshi Uemori

Wako Pure Chemical Industries, Ltd. Reagent Research Laboratories 6-1, Takadacho, Amagasaki, Hyogo Pref. 661-0963, Japan

Abstract:

Recently the food additive and the veterinary product that remains in food became a problem. Tetracyclines are one of such substances and analyzed by HPLC-fluorescence detection method. The extraction samples of foods were pretreatmented by solid phase extraction column (styrene divinyl benzene copolymer packed column). Pretreatment of the shrimp extracts was investigated. The simple two step extraction with Presep-C RPP(styrene divinyl benzene copolymer packed column) and Presep-CM (cation exchange column) improved effect of the cleanup and recovery rates of Tetracyclines efficiency compared with one step of styrene divinyl benzene copolymer packed column.

Keywords: HPLC, Solid phase Extraction, Tetracycline

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