CHROMATOGRAPHY, Vol. 26 (2005), No. 2, pp. 57-58
Technical Review
High Speed Gradient Analysis Using a Reversed-Phase Packed Column
Miho Ebata1, Yoshihisa Hiroe2, Hiroko Arai1, Hiroshi Kutsuna2 and Osamu Shirota1
1Shiseido LC Application Lab., 2-2-1 Hayabuchi, Tsuzuki, Yokohama 224-8558, Japan
2Chromatographic Research Lab., 2-2-1 Hayabuchi, Tsuzuki, Yokohama 224-8558, Japan
Abstract:
Ultimate performances of short packed C-18 columns were studied to meet the current high-throughput trend. Using a short Capcell Pak
MGⅡ S 3 column (2.0 mm I.D. x 20 mm), retention behaviors of seven standard sulfa drugs were examined under gradient elution with increased
flow rates. After the optimization, it was possible to shorten a run time to 1.7 min with base-line separations among the seven peaks.
The gradient system was also applied to peptide mapping in LC. A tryptic digest of human albumin could be run at a run time of 2.5 min.
