CHROMATOGRAPHY, Vol. 26 (2005), No. 1, pp. 11-21

Capillary zone electrophoresis has been utilized for the analysis of chemical equilibria in aqueous solution. Electrophoretic mobility of analyte changes along with the addition of interacting reagent in the migrating solution, and precise measurement of the electrophoretic mobility allows analyzing equilibrium reactions. The capillary electrophoretic method including mathematical analyses was validated through complex formation reactions, and it was applied to the investigation of ion association reactions. Various types of interactions have been clarified; contributions of hydrophobicity, probability, and aromatic.aromatic interaction were quantitatively evaluated. Ion association reaction in aqueous solution also elucidated the stepwise reactions of liquid.liquid distribution of ion associates. The technique was further developed to the binding analysis of ions and ion associates to nonionic surfactant micelles.