Focussing Review

Development of Highly Selective Analytical Systems for Biological Substances Using Chromatography Combined with Mass Spectrometry
--With Special Reference to Bio-Analytical Studies of Bile Acids--

Junichi Goto^1,2*, Nariyasu Mano² and Takaaki Goto²

¹Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan
²Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, 980-8578, Japan

Abstract:

For the diagnosis of disease, reliable analytical methods for biologically important compounds are essential. Due to the existence of these compounds in complex matrices, such as blood, urine, or tissue, chromatographic procedures are preferable. In this article, we review highly selective and sensitive chromatographic methods coupled with mass spectrometry for the analysis of biologically important materials, especially bile acids and related compounds. We also discuss immunoaffinity extraction employing highly specific antibodies.

Keywords: gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry, immunoaffinity extraction, bile acid, drug, biological material, stable isotope labeled compounds, immobilized antibody

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