CHROMATOGRAPHY, Vol. 24 (2003), No. 2, pp. 81-87
Original
Extraction Method with High Boiling Solvent for Camptothecin and
Its Analogues in Camptotheca acuminata, and Rapid HPLC Analysis
with Monolithic Column.
Masako Nakazawa, Hiroshi Hatano, Masato Nagaoka and Ritsuo Aiyama
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi.shi, Tokyo, 186-8650, Japan
Abstract:
For determining camptothecin and its analogues in several parts of a Chinese tree, Camptotheca acuminata, a small.scale extraction method
with a high boiling solvent such as 2.methoxyethanol and a high throughput analysis using a monolithic column were developed. A number
of plant samples were simultaneously extracted in a small scale and the contents of the constituents in the extracts were measured within 4
min by this HPLC method.
Keywords:
camptothecin ,HPLC, monolithic column, 2.methoxyethanol, extraction
