Focussing Review

Development of Analyses of Biological Steroids Using Chromatography --Special Reference to Vitamin D Compounds and Neurosteroids--

Kazutake Shimada*, Tatsuya Higashi and Kuniko Mitamura

Faculty of Pharmaceutical Sciences, Kanazawa University, 13-1 Takara.machi, Kanazawa 920-0934, Japan

Abstract:

Steroids comprise a large group of natural substances that must frequently be monitored in various biological materials. Due to the metabolic versatility of steroid molecules, extremely complex mixtures are often encountered, necessitating the use of a chromatographic procedure prior to measurement. In this article we present our work, that is, the development of analyses of biological steroids (especially vitamin D compounds and neurosteroids) using gas chromatography/mass spectrometry, high.performance liquid chromatography (including inclusion chromatography using cyclodextrin) and liquid chromatography/mass spectrometry.

Keywords: estradiol, guaiacol estrone, rat brain, standard addition method, gas chromatography/mass spectrometry/mass spectrometry

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