|
Original
Studies on Polyester Chips with Modified Inner Surface
for Open.Channel Electrochromatography
|
|
Wei Xu+, Katsumi Uchiyama* and Toshiyuki Hobo
|
|
Faculty of Pharmaceutical Sciences, Kanazawa University, 13−1 Takara−machi, Kanazawa 920−0934, Japan
|
Abstract:
Modification of polyester microchip inner surface was carried out through incorporation of functional moieties into polyester matrix during
chip fabrication procedure. Olefin alcohols with different alkyl chain length were chosen to serve as stationary phases for open.channel electro.
chromatography. The modified polyester microchannels exhibited a high.resolution efficiency. The 10.undecene.1.ol modified polyester
chip was evaluated in terms of its mass loadability, effect of chain length of olefin alcohols, effect of the content of 10.undecene.1.ol, effect
of buffer pH, effect of methanol content in running buffer, and reproducibility. The study on van Deemter plot revealed the factors resulting
in band broadening in 10.undecene.1.ol modified polyester chips. The considerable large retention of samples on 10.undecene.1.ol
modified polyester channels showed that 10.undecene.1.ol plays a significant role in separation. The migration order of Rhodamine B could
be regulated by the content of 10.undecene.1.ol in the polyester matrix. The separation of laser dyes was based on the hydrophobic.hydrophobic
interaction, while static interaction between charged components and negatively charged channel walls also cannot be excluded.
|
| Keywords: |
microchip, electrophoresis, open.channel chromatography
|
|
|
+Currently working at Waseda University as a postdoctoral fellow
|
|
|
|
