Endotoxin Removal in Some Medicines and Human Serum Albumin Solution by Affinity Membranes
|Wei Gui-lin, Liu Xue-liang*, Li Jing-hua, Liu Ying and Shang Zhen-hua|
|Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China|
Three kinds of affinity membranes utilized for endotoxin removal, namely, chitosan affinity membrane (KFCC 517), hydrophobic and cationic
charged membrane (KFCG 316) and metal chelate affinity membrane (KFCM 402) were prepared by using cellulose membrane as support.
The adsorbability and applications of the membranes in endotoxin removal were investigated. The results showed that all of these affinity
membranes could be utilized for endotoxin removal in several kinds of solutions. The factors affecting endotoxin removal such as ionic
strength, pH value and flow rate were investigated, and the results were discussed. Three cartridges of membranes KFCC 517, KFCG 316 and
KFCM 402 were utilized for endotoxin removal in human serum albumin solution. The cartridge of membrane KFCC 517 also was utilized in
medicine injections, such as hydrocortisone, puerarin, tetracaine hydrochloride and dextran 40 glucose. Under the optimal conditions, the removal
efficiencies of endotoxin were over 80% and the recoveries of desirable substances were over 95%.
|Keywords: ||endotoxin, removal, affinity membrane, human serum albumin, medicine|