CHROMATOGRAPHY, Vol. 22 (2001), No. 3, pp. 201-207

Protein Functional Analysis Using Liquid Chromatography −Tandem Mass Spectrometry in Post-genomic Era
Hiroshi Nakayama
Biomolecular Characterization Division, RIKEN (The Institute of Physical and Chemical Research)

Ways to identify functions of a biomolecule from its structure is important for biology in post-genomic era because extensive DNA sequencing has been accumulating vast amounts of information on genes/proteins without functional annotations in databases. To understand protein function, protein chemical analysis or its descendant ‘proteomics’, together with genomic and genetic researches, must be used because of no strict linear relationship between genome and the protein complement or ‘proteome’. Here the author describes his and other researchers’ efforts applied to three important classes of protein functional analysis from methodological viewpoint: biomolecular interaction analysis, posttranslational modification analysis and proteomic profiling.
Keywords: proteomics, biomelecular interaction, post-translational modification, proteomic profiling, microfluidics

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