CHROMATOGRAPHY, Vol. 22 (2001), No. 3, pp. 195-204

Voltage-induced Variation of Capacity Factors for Neutral Solutes by Using Hexa-6-bromo-hexa-6-deoxy-β-cyclodextrin Modified Column in Electrochromatography
Masahiko Sato, Shinya Kitagawa*, Hiroyuki Tashita and Takao Tsuda
Department of Applied Chemistry, Nagoya Institute of Technology Gokiso, Showa, Nagoya 466-8555, Japan

The voltage-induced variation of capacity factors of neutral compounds in pressurized flow driven capillary electrochromatography were investigated. The capillary column packed with hexa-6-bromo-hexa-6-deoxy-β-cyclodextrin modified silica gel and neutral solutes were used. The capacity factors of four neutral solutes were able to vary with the application of voltage. All solutes took maximum capacity factors with the application of -3 kV. The voltage-induced variation of relative capacity factor did not depend on the kind of solutes. The variation of the column conductance was also observed. The comparison between hexa-6-bromo-hexa-6-deoxy-β-cyclodextrin and ODS modified columns suggested that the surface charge of the packing material is one of the key factor of the voltage-induced phenomena. The direct control of the retention behavior might be possible by using application of the voltage in same extent.
Keywords: electrochromatography, capacity factor, conductance, electric double layer, β-cyclodextrin, ODS

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