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Original Paper
Retention Behavior of Reversed-Phase for HPLC Using 100% Aqueous Mobile Phase(2) |
| Toshiyuki Enami and Norikazu Nagae |
| Nomura Chemical Co., Ltd., 851 Anada-cho, Seto, Aichi 489-0003, Japan |
Abstract:
The retention behavior of the reversed-phase was evaluated under 100% aqueous conditions. It is commonly said that reversed-phases, such as ODS (C18) and C8, show a decrease in the retention time under 100% aqueous conditions. It was found that 100% aqueous mobile phase was pulled out of the pore of the packing materials, so that the stationary phase in contact with the mobile phase decreased and the retention time decreased. Some parameters, such as the pore size, length of the alkyl group of the stationary phase, the amount of residual silanol groups of the stationary phase and back pressure of the column, were shown to influence of the decrease in retention.
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| Keywords: | HPLC, reversed-phase, 100% aqueous mobile phase, retention behavior |
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