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Mutual Citation
Cibacron Blue F3GA-Attached 2 µm Non-Porous Monodisperse Silicas for Affinity Chromatography |
| Shen Li, Xiong Bohui, Cong Runzi and Wang Junde |
| Dalian Institute of Chemical Physics, The Chinese Academy of Science, Dalian 116012,China. |
Abstract:
Non-porous monodisperse silica (NPS), 2 µm in diameter, was modified with 3-aminopropyltriethoxysilane for immobilized of Cibacron Blue F3GA, a packing of NPS-ACB for high performance affinity chromatography was obtained. Up to 2 mg of Cibacron Blue F3GA could be attached to 1 ml of NPS beads. There was no obvious leakage of dye from NPS-ACB. The adsorption of lysozyme varied with pH value and ionic strengths. Furthermore, the column could be used for separation and preparation of lysozyme from hen egg white. In addition, rapid separation of α-, β-,γ-globulin is also described.
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| Keywords: | high performance affinity chromatography, non-porous monodisperse silica, Cibacron Blue F3GA, lysozyme, α-globulin, β-globulin, γ-globulin |
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