CHROMATOGRAPHY

CHROMATOGRAPHY, Vol. 21 (2000), No. 3, pp. 217-220
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Mutual Citation
Beaded molecule imprinted polymer for stereo isomer separation
Meng Zihui 1), Wang Jinfang 2), Zhou Liangmo 2), Wang Qinghai 2) and Zhu Daoqian 2)

1) Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, P.R.China.
2) Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116012, P.R.China.


Abstract:
Beaded Molecule imprinted polymer (MIP) for cinchonan-9-R-ol (cinchonidine) was prepared by suspension polymerization. Particles with the size of 50-70 mm in diameter were collected and evaluated in HPLC mode to separate stereo isomers. Stereo isomers cinchonine and cinchonidine were successfully discriminated with selectivity factor of 2.89 and resolution factor of 0.76. Stereo selectivity of the MIP was found to come from both the interactions between the analyte and carboxyl group on the MIP and the complementary between the stereo structure of imprinted molecule and the MIP. The thermal analysis results showed that the MIP had high thermal stability with initial thermal decomposition temperature of 320 . The pore volume of MIP was 0.1849 mL/g, the specific surface area was 126.84 sqm/g and the average pore diameter was 5.8 nanometer. Scanning electron microscropy showed that MIP had perfact spherical morphology.
Keywords: molecule imprinting, suspension polymerization, stereo isomer


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