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Original Paper
Development of Separation Method of Ginseng Saponins using Ion Exchange Resin |
| Jong-Hwan Sung1), Hee Jeong Chae2) and Eui Yong Kim |
Department of Chemical Engineering, University of Seoul, Seoul 130-743,Korea
1) Central Research Institute, IL-HWA Co.,Ltd., 471-030, Korea
2) Department of Food Technology, Hoseo University, Asan 336-795, Korea |
Abstract:
A new method to prepare saponin fractions from Ginseng whole extracts was developed. A separation procedure was establisherd using HP-20, a porous adsorption resin. After adsorption, fellowed by elution with water and methanol, high yield and high content of Ginseng Total saponins (GTS) were obtained. The recovery yields of GTS prepared by conventional methods such as n-butanol (BuOH) method and sep-pak C18 cartridge method were compared with that by HP based method. Ginsenoside content in GTS prepared by HP method was significantly higher than that by BuOH-method. In addition, when HP method was used, GTS was separated completely into protopanaxadiol (PPD)- and protopanaxatriol (PPT)-type saponins with high rapidity. Consequently HP-20 method was found to be superior to BuOH method in the preparation of GTS, PPD and PPT fractions from Ginseng whole extracts.
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| Keywords: | Ginseng total saponins(GTS), HP resin, Protopsnaxadiol(PPD), Protopanaxztriol(PPT), Recovery yield |
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