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Focussing Review
Electrochromatography using in-capillary polymerized separation media |
| Chuzo Fujimoto |
Department of Chemistry, Hamamatsu University School of Medicine
Hamamatsu, 431-3192, Japan |
Abstract:
The objective of this review is to describe column technology in capillary electrochromatography (CEC), focusing especially on the methods for the in situ preparation of separation media inside fused silica capillaries. There are many monomers which can be polymerized directly in a capillary to be used as stationary phase for CEC separation: (comonomers) acrylamides, alkyl acrylates, and styrene; (crosslinking monomers) diacrylamides, diacrylates, and divinylbenzene; and (functional monomers) 2-acrylamido-2-methyl-1-propane sufonic acid, vinyl sufonic acid, acrylic acid, acrylamido ammonium halides, and ally ammonium halides. In addition to the organic polymer-based stationary phases, silica-based stationary phases are prepared from teraethoxysilane or tetramethoxysilane by the sol-gel method. In situ prepared columns possess some desirable features: column packing and frit construction - the method makes these difficult steps unnecessary; and the electroosmotic flow is expected to be constant at every part of the column. Fifty-three papers are cited to review the development in the specific area.
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| Keywords: | capillary electrochromatography, fritless columns, monolith, rod, stationary phases, electroosmotic flow |
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