CHROMATOGRAPHY, Vol. 35 (2014), No. 1, pp. 23-29
Development of the High Sensitive Separation Analysis Method of Metabolites in Human Nail and its Application to the Diagnosis of Chronic Disease
1Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan
2College of Pharmacy, Yanbian University, Yanji City 133002, Jilin Province, Peoplefs Republic of China
This review summarizes the possibility to diagnosing the chronic diseases and application of determination of metabolites of the noninvasive human nails. A target derivatization UPLC-ESI-MS method is a powerful tool for the analysis of bioactive compounds with high sensitivity and selectivity. We developed target derivatization UPLC-ESI-MS methods to analyze the biological trace components in human fingernail. Moreover, we have used these methods to detect the metabolites in human fingernails, such as DL-amino acids, polyamines and Advanced Glycation End products (AGEs). Through detecting the biological trace components in the real samples from human, we tested the possibility to use these new methods to diagnosing the chronic diseases, i.e., diabetes, lung cancer. Some applications utilizing these target derivatization methods for the analyses determination of metabolites in the human fingernails are also described in this paper.
Human nail, Target derivatization, Diabetes, Lung cancer, UPLC-ESI-MS.
Received: 3 January 2014
Accepted: 24 January 2014