CHROMATOGRAPHY
CHROMATOGRAPHY, Vol. 34 (2013), No. 1, pp. 1-22
Focusing Review
Simple LC using New Macroporous Polymers
Ken Hosoya
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
1-5 Shimogamo-hangi-cho, Sakyo-ku, Kyoto 606-8522, Japan
Abstract:
New macroporous polymers have been prepared based on two different “platforms” to simplify rather complicated pretreatment processes for LC analyses. For example, uniformly sized, molecularly imprinted polymer particles modified with sulfonic acids on outer surface easily removed humic acids in environmental water sample to improve detectability of really low level of the target molecule. Nicely controlled, 3D skeletal co-continuous polymers (monolithic polymer) have been prepared using visco-elastic phase separation mechanism as well as the utilization of epoxy monomers and a diamine. In addition, a hydrophilic monolithic media based on ethylene oxide units simplified affinity chromatography through avoiding non-specifically bound proteins even if those were utilized in protein lysate. All the macroporous polymers have contributed to simple and accurate LC analyses.
Keywords: Macroporous polymer, HPLC, Selectivity, Monolithic media.