CHROMATOGRAPHY, Vol. 33 (2012), No. 1, pp. -19

Fluorogenic derivatization (FD)-HPLC is a powerful tool for analyzing biological samples due to its high sensitivity and reproducibility. In recent years, we have developed FD-LC-MS/MS, a novel proteomics method that consists of HPLC separation of fluorogenic derivatized biological macromolecules with DAABD-Cl and identification of the separated derivatives. To develop the analytical system for differential analysis, high-resolution HPLC conditions were optimized. The analytical system was then developed to clarify the flow dynamics of signaling in cells. The affinity column for extracting albumin in plasma was also evaluated. FD-LC-MS/MS method is superior to other proteomics methods in sensitivity, quantitativity and reproducibility, and enables demonstrating flow dynamics in cells.