Focusing Review

NanoLC-MS systems in proteomics

Yasushi Ishihama

Institute for Advanced Biosciences, Keio University and PRESTO, Japan Science and Technology Agency, 403-1 Nipponkoku, Daihoji, Tsuruoka, Yamagata 997-0017, Japan

Abstract:

Current proteomics technologies based on mass spectrometry coupled with miniaturized on-line liquid chromatography allow us analyzing tryptic peptides for protein identificaiton with high throughput. Compared to genome sequencing and microarray-based transcriptomics, however, proteomics is still under development to achieve the complete coverage of whole proteome including protein expression profiles, protein -protein interaction and post-translational modifications. In this review, the current status of proteomic nanoLC-MS/MS systems is addressed. In addition, the phosphorylation-focused proteome analysis systems consisting of phosphopeptide enrichment methods and nanoLC-MS is presented as an example of post-translational modification proteome. Furthermore, the future prospects for proteome analysis systems are discussed.

Keywords: nanoLC, LC-MS, proteomics, phosphoproteome, post-translational modification, omics

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