CHROMATOGRAPHY, Vol. 28 (2007), No. 2, pp. 81-87
Technical Review
High−Speed Analyses using Rapid Resolution Liquid Chromatography on ZORBAX column packed 1.8 μm Particles
Tatsunari Yoshida(1), Ronald E. Majors(2) and Hiroki Kumagai(1)
(1) Agilent Technologies, Inc. 9−1 Takakura−Cho, Hachioji−Shi Tokyo, 192−8501 Japan
(2) Agilent Technologies, Inc 2850 Centerville Road Wilmington, Delaware 19808 USA
Abstract:
The potential of high−speed analyses by Rapid Resolution Liquid Chromatography (RRLC) and RRLC/mass spectrometry (MS) on 1.8 μm
porous particles packed into short columns operated at high flow rate was investigated and compared to the performance of 5 μm porous particles packed into conventional columns. Using similar chemistries, the ease of conversion from conventional HPLC to RRLC method was demonstrated. In order to display the practicality of RRLC separations, the isocratic analysis of pesticides and the gradient analysis of catechins in Japanese Green Tea were selected. In isocratic analysis, compared to the conventional method, the time could be reduced by a factor of 31 at the highest flow rate. In gradient elution, the fastest separation was achieved by RRLC on 50 mm×4.6 mm i.d. or ×2.1 mm i.d. RRLC column packed 1.8 μm particles. The analysis time was reduced by a factor of 15, compared to the conventional method.
Keywords:
Rapid Resolution Liquid Chromatography, High throughput, Fast LC, sub−micron, 1.8 μm, pesticide, cathechin
