CHROMATOGRAPHY, Vol. 24 (2003), No. 3, pp. 117-120
Evaluation of Accuracy for the Quantitative Analysis
Using Nuclear Magnetic Resonance as a Detector of HPLC
National Metrology Institute of Japan, AIST
Higashi 1-1-1, Tsukuba Central 5-2, Tsukuba, Ibaraki 305-8565, Japan
Continuous.flow LC.NMR technique was applied for the purity assessment of o.xylene. Two consecutive LC.NMR runs of a sample and a
standard were measured. The purity was calculated by the ratio of the set of data. With such an approach, sources of NMR problems were
overcome because identical condition can be obtained for both the standard and the sample during NMR measurements. As a result, significant
reduction of dispersion and considerable improvement of accuracy in the results were obtained compared to the previous work reported.
Comparison of the result of purity assessment obtained with LC.NMR to GC.FID and differential scanning calorimeter (DSC) showed excellent
agreement. With such an NMR experimental condition, on.flow NMR can provide accurate and precise results as a detector of HPLC.